Keywords :
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Microbial, Dissolution, Destruction, Cariogenic, Sustainable, Environment friendly.
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Abstract :
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Healthy teeth and gums are an important part of one’s health and appearance. Streptococcus mutans is known as the causative bacteria in the formation of dental plaque and dental caries. There are many benefits of herbal drugs such as they have long era of use and better patient tolerance as well as public acceptance, they act as a renewable source which is our only hope for sustainable supplies of cheaper medicines for the world’s growing population, the cultivation and processing of medicinal herb and herbal products are environment friendly. This study was done to prevent the use of chemicals and to provide knowledge of leaves of Aloe vera and Ocimum sanctum. Bacterial sample was collected from saliva and dental plaque. Estimation of Streptococcus mutans was done using sheep blood agar medium, Mitis salivarius bacitracin agar medium and gram staining. Confirmatory tests like catalase and carbohydrate fermentation were also performed MIC was done using serial dilution method and brain heart infusion was taken as supplement media. Bacterial isolates were identified as positive Gram stained catalase negative, spherical or ovoid shape, negative growth at pH 9.6, alpha haemolytic, Bacitracin resistant and sucrose positive. Aqueous and alcoholic extract of Aloe vera and Ocimum sanctum leaves was prepared at concentration of 10%-50% and 1-10% respectively to observe minimum bacterial concentration. Comparable work on both aqueous and alcoholic extract of Aloe vera and Ocimum sanctum leaves showed that alcoholic extract of Ocimum leaves is most effective at 6%.
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