Keywords :
|
Microbial, Dissolution, Destruction, Edentulous, Ubiquitousness, Haemolytic.
|
Abstract :
|
Dental caries is an infectious microbial disease that results in localized dissolution and destruction of the calcified tissues of the teeth (Loesche WJ., 1986). Dental caries can also cause bad breath and foul tastes. In highly progressed cases, infection can spread from the tooth to surrounding soft tissues which may lead to an edentulous mouth ( Baelum .V et al., 1997) . Dental caries is one of the most important problems in public health because of its ubiquitousness in civilized populations. The association of Streptococcus mutans with dental caries was first reported by Clarke (1924) who isolated the organism, frequently in pure culture, from the depth of carious lesions. This study was done to check the use chemicals and to provide the knowledge of the use of Punica granatum and Ocimum sanctum leaves. Bacterial sample was collected from saliva and dental plaque. Estimation of Streptococcus mutans was done using sheep blood agar medium, Mitis salivarius, bacitracin agar medium and gram staining. Confirmatory tests like catalase and carbohydrate fermentation were also performed MIC was done using serial dilution method and brain heart infusion was taken as supplement media. Bacterial isolates were identified as positive Gram stained catalase negative, spherical or ovoid shape, negative growth at pH 9.6, alpha haemolytic, Bacitracin resistant and sucrose positive. Aqueous and alcoholic extract of Punica granatum and Ocimum sanctum leaves was prepared at concentration of 10-50% and 1-10% respectively to observe minimum bacterial concentration. Comparable work on both aqueous and alcoholic extract of Punica granatum and Ocimum sanctum leaves showed that alcoholic extract of Ocimum sanctum leaves is most effective at 6%.
|