Title :
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Isolation, Characterization and Optimization of Cellulase Producing Fungi for Maximizing the Enzyme Yield
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Author :
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Shelly Rana, kamaldeep, Manjeet Kaur
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Journal name :
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IJMRS's International Journal of Engineering Sciences, ISSN 2277-9698
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Volume :
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Volume 01, Issue 03, Sep. 2012
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Keywords :
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cellulase, optimization, 18S rRNA.
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Abstract :
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The main objective of this study is to isolate the novel Cellulase producing microorganisms. The enzyme production rate from fungi is higher as compared to other microorganism. For this study soil samples were collected from wood and cellulosic waste collected from dumping areas. Microorganisms were isolated using Potato dextrose agar medium. Presently, the work is aimed at identification of the isolates based on staining and molecular characterization and optimization of the cultural and environmental conditions for maximizing the yield of the enzyme. Identification of the fungal isolates was done based on biochemical and molecular characterization by sequencing the 18S rRNA coding gene. Crude enzyme was extracted and purified by centrifugation. The optimization of various different parameters of the mediumwas carried out to maximise the yield of cellulase.
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